09/12/2018- Soil Metadata analysis and Spot test

09/12/2018

Soil Metadata Analysis and Spot test

Research Question:

Does the presence of arthrobacter appear more dominant in the soil of one oak species than the others? Is there a correlation between the presence of Arthrobacter Phage and the presence of oak wilt fungus?

Objectives:

calculate % water, % sand, %silt and % clay.
make spot test using the lysate extracted from soil sample B

Materials required:

micropipettes, agar plates, micro centrifuge tubes, soil metadata collection samples prepared on 09/10/2018, 50 ml conical vials, serological pipettes, 1 M CaCl2 , phage buffer, enriched lysate, direct isolation lysate, 0.5 ml of arthrobacter, 2X top Agar, LB broth, syringe, syringe filters ( 22 microns).

Procedure:

Soil Metadata:

% Water:

collected the soil that was put in the weighing boat outside to dry.
massed the dry soil and boat on the scale
subtracted the mass of the boat from the total weight( boat and dry soil)
using % water = mass of dry soil / mass of wet soil x 100, the percent water was calculated

Calculations

mass of boat = 2.330 g

mass of wet soil and boat= 8.700g

mass of wet soil= 8.700-2.330= 6.370g

mass of dry soil and boat = 7.929g

mass of dry = 7.929-2.330= 5.599g

% water= mass of wet-mass of dry/ mass of wet x 100= [( 6.370-5.599)/6.370] x 100= 12.1%

% sand, silt and clay

retrieve falcon tube with soil sample for collecting metadata on the type of soil
record the level to which there sand on the bottom
do the same for silt and then clay
the total for all was 10 ml.
calculate % sand silt and clay as done below.

Calculations :

amount of sand= 7ml

amount of silt= 0.5 ml

amount of clay= 2.5 ml

total = 10 ml

% sand = amount of sand / total x 100 = 7/10 x 100 = 70%

% silt= amount of silt / total x 100 = 0.5/ 10 x 100 = 5 %

% clay= amount of clay/ total x 100 = 2.5/10 x 100 = 25 %

Type of Soil : loam

based on chart below

Spot test:

Calculations:

conversion factors:

1M= 1000mM

1 ml =1000 microliters

M1V1=M2V2

1M(V1)=(4.5mM)(10ml)

1000mM(V1)=(4.5mM)(10000 microliters)

V1=45 microliters

Procedure:

First the aseptic zone was set up: pour Cidecon on the desk and wiping it till the desk dry. then pour ethanol and wipe it all over the table and let it evaporate.
Light the ethanol lamp to create an air current near which the samples can be opened to prevent things from getting into the samples.
make on TA for the group. because four plates are made ( 1 per person and control), adjust amounts accordingly
Retrieved the LB broth, a 50 ml conical tube and a serological pipette
While in the aseptic zone, transfer 8 ml of LB broth to the 50 ml conical vial.
now retrive the 1 M CaCl2 stock solution.
Using the micropipette, i transfered 90 microliters of the CaCl2 to the 50 ml conical tube with the LB broth.
retrieved 0.5 ml of arthrobacter from Lathan ( Teaching Assistant)
add the arthrobacter to the 50 ml conical tube.
add 10 ml of top agar to the 50 ml conical tube.
pour the contents of the 50 ml conical tube onto the agar plate.
to let the top agar solidify, i waited for 10 minutes.
collect the enriched sample tube, a syringe and a filter of 22 microns.
take a sample from the enriched tube using the syringe.
attach filter to the syringe and pour the lysate out slowly into a microcentrifuge tube
collect the direct isolation sample from the fridge.
collect a phage buffer from the instructor
mark the agar plate with three spots , one for the enriched, one for the direct and one for the phage buffer.
spot 10 microliters each of the phage buffer, enriched sample and the direct isolation sample.
let the sample rest for 10 minutes
i put the plate in the incubator, where it will remain for 48 hours.

Analysis:

there was no event that may have caused contamination to the sample. the aseptic zone was properly maintained. the procedure was properly followed.

Future notes:

take more pictures to have a proper record of all set ups.

Pictures:

Category: Dr. Adair, Uncategorized | LEAVE A COMMENT

September 13

9/12/18 Spot Test/Plaque Assay/Continuation of Soil Metadata

Rationale: Isolate bacteriophage through both a Spot Test and Plaque Assay., and to collect more metadata soil sample B.

Procedures:

Before starting we had to create an aseptic zone to ensure that all bacteria were killed, and the working space would not contaminate our experiments.

Cleaned off the workspace with CiDecon and applied the table with 70% ethanol solution.
Wiped off the table after CiDecon was applied, same with the 70% ethanol solution, only, we let the ethanol solution evaporate.
We then got an ethanol burner, and our aseptic zone was created.

Spot Test and Plaque Assay

Spot Test

The enriched lysate was made on 9/10/18.
Measured the mass of the enriched lysate. Mass of enriched lysate: 20.47 g.
The enriched lysate was spun in the centrifuge for 5 minutes at 3000 x g.
The enriched lysate was filtered and put into a small cap.
Divided one plate into four quadrants
- I – ML Spot Test
- II – CJJ Spot Test
- III – JLL Spot Test
- Buffer
Added 2mL LB broth into a 50mL vial.
Added 22.5 microliters of M Calcium Chloride in the 50mL vial.
Added 0.5mL OF Artho PA Phage lysate
Added 2.5 mL TA into the 50mL vial.
Poured our solution onto the one plate that is divided into four quadrants.
Sat the petri dish aside to let TA solidify (1o minutes).
Added 0.5 microliters directed (filtered) lysate on top of the TA.
Added the buffer onto the last quadrant, our negative control.
After all the quadrants were filled with a spot, we placed the plate in the incubator.

Plaque Assay

Got four different petri dishes, one for each lab partner, and the fourth one being our control buffer.
In each dish we added:
- Added 2mL LB broth into a 50mL vial.
- Added 22.5 microliters of M Calcium Chloride in the 50mL vial.
- Added 0.5mL OF Artho PA Phage lysate
- Added 2.5 mL TA into the 50mL vial.\
  - Note this times 4 since we had to fill 4 petri dishes.
We then separated the solutions into 15mL vials and we added our 0.5 microliters of direct lysate into the 15mL vial.
Poured the Solution onto my petri dish labeled ML 9/12/18 Plaque Assay.
Let the solution sit for 15 min and placed in the incubator.
Cleaned off the workspace with CiDecon and applied the table with 70% ethanol solution.
Wiped off the table after CiDecon was applied, same with the 70% ethanol solution, only, we let the ethanol solution evaporate.

Soil Metadata

Percent water of the soil was calculated to be 11%.
- Mass Dry= 10.8
- Mass Wet= 12.09
- 10.8/12.09=.89
- 1-.89=.11=11%
  - Side note: Lots of debris which could have an effect on the % water.

The mass of the soil was recorded and left to sit under a vented hood in the lab (this was done 9/10/18). This allowed the procedure above to be done today in lab.
% Soil, Clay, and Silt was then calculated
50mL vial was left under a vent hood from Monday to let the soil disperse.
The vial was observed and these are the results.
- 1.5mL Sand
- .5mL Clay
- 2mL Silt

Observations:

The soil had a lot of debris in both the % water mass and the vial shown above which was used to measure % soil.
Results from the metadata:
- 11% Water
- 1.5mL Sand
- .5mL Clay
- 2mL Silt
- 6.5pH

Conclusions/Next Steps:

The overall spot test and plaque assays procedures were easy since we knew what to expect. We were better prepared versus last time. Next steps: to see if our Bacteriophage has been isolated, and if so, we will try to amplify it.

Category: Michael Lum, Uncategorized | LEAVE A COMMENT

September 11

9/10/18 Soil Washing and Enrichment and Metadata Soil B

Rationale: Isolate possible bacteriophage through the process of soil washing and enrichment. The soil metadata will also be found and recorded.

Soil Washing

Before starting we had to create an aseptic zone to ensure that all bacteria were killed, and the working space would not contaminate our experiments.

Cleaned off the workspace with CiDecon and applied the table with 70% ethanol solution.
Wiped off the table after CiDecon was applied, same with the 70% ethanol solution, only, we let the ethanol solution evaporate.
We then got an ethanol burner, and our aseptic zone was created.

Procedure:

10mL LB broth was added to my 15mL vial, which contained 2mL of Soil B, giving a total volume of 12mL solution in my 15mL vial.
Started to shake my 15mL for 11 minutes.
Measured the mass of the 15mL vial which read 14.44g.
Found a partner that had .05+/- difference in mass for the centrifuge.
Spun the 15mL solution at 10,000g f0r 10 minutes.
Top filtered the solution after it was spun in the centrifuge, which came to about 7mL of my enriched lysate.
Then placed my 7mL solution into the Cabinet.

Observations:

The soil wash was not hard at all compared to my first soil wash.
The only hard part was the filtering process since I had a lot of debris on the top of my enrichment.
7mL of enriched lysate was made.

Soil Metadata (% Soil, Clay, and Silt. pH of Soil. % Water.)

While the Soil Wash tubes were being shaken, the soil metadata was collected.

Procedure %Water:

The procedure to calculate the percent water started with pouring soil B from the plastic bag to the petri dish.
Mass of the weight boat = 2.39g.
Mass of weight boat with soil = 14.48g.
The weight boat was then labeled ML 9/10/18 14.48g.
The weight boat was then placed under the vented hood, where it will sit for 48 hours to allow the water to evaporate.

Procedure for pH of the soil:

Poured a small amount of soil into a pH vial.
Filled rest of the vial with DI water.
Rested my hand over the top, and I shook the vial for about 45 seconds.
Put the short strip (<1 inch) pH paper into the vial for 45 seconds.
Quickly compared the pH paper to the pH scale (<1 min).
pH recorded

Procedure for Metadata:

Filled 4mL of soil B into a 50mL vial.
Added 8mL of DI water into the 50mL vial. Total 12mL solution.
Added three drops of soil dispersion liquid into the 50mL vial solution.
Shook the vial for 45 seconds, with my hand covering the top (no lids were available).
Labeled the vial ML Soil B 9/10/18
The vial was then placed under the vented hood, where it will sit for 48 hours to let the soil (clay, silt, and soil) completely separate.

Observations/Results:

This metadata procedure was not hard. The only hard part was not to get any debris into any of the tests, which my soil B had a lot of debris. This could have an effect on all of my tests.
pH of soil: 6.5
Mass of weight boat: 2.39g
Mass of weight boat with soil: 14.48g

Next steps/Conclusions:

Prepare for a Spot Test/Plaque Assay and the continuation of Soil metadata results. Find the mass of water from the weight boat by doing a simple calculation. Find the composition of soil by observing the results in the 50mL tube. The next steps will be done 9/12/18. The spot test should not be hard, and hopefully, the simple calculations are not done incorrectly.

Category: Michael Lum, Uncategorized | LEAVE A COMMENT

September 11

09/10/2018- Washing And Enrichment of Soil B

09/10/2018

Washing And Enrichment of Soil B

Research Question:

Objective:

Prepare direct and enriched lysate for soil sample B
Collect metadata for soil sample B ( %water, pH, and sand silt clay percentages)

Materials Required:

50 ml conical vials, 15 ml conical vials, pH vials, pH paper, serological pipettes, Cidecon, 70% ethanol, LB broth, Soil Sample B, Falcon tubes, DI water, soil dispersion liquid, scooper, syringe, syringe tip filter (22 microns), shaking incubator, weighing boat

Procedure

set up an aseptic zone by cleaning your desk with Cidecon (wipe till dry) and ethanol ( 70%)( wipe on the desk and let it evaporate) after clearing the table.
lit the ethanol lamp to set up an air current to help keep other microbes from getting into tube when it is open.

Washing and Enrichment:

retrieved Soil Sample B from the refrigerator.
retrieved the 15 ml conical vial with 2 ml of soil in it.
retrieved the LB broth
using a serological pipette, transferred LB broth to the 15 ml conical vial until it reached the 11 ml mark.
closed the 15 ml conical vial and shook it by hand for 12 minutes
after 12 minutes, find the mass of the vial ( 19.31g), and find another lab partner with a tube of mass within 0.05g of your tube mass.
take the vials to the centrifuge and put them in for 10 minutes at an acceleration 10000 times g.
take the vial back to the aseptic zone.
using a sterile syringe and a syringe tip filter (22 microns), transfer 10 ml of filtered lysate to a 50 ml conical vial. ( take care to use the aseptic zone to avoid contamination of lysate.
after you acquire 10 ml of filtered lysate, add 0.5 ml of arthrobacter to the vial. this is the enriched sample.
place this tube, loosening the cap a little bit , into the shaking incubator for 48 hours
filter the rest of the lysate into another 15 ml conical vial ( 1/2 ml for my sample). this will be your direct isolation sample
store direct isolate in the fridge

% Water

weigh the weighing boat on the scale ( g)
pour some soil onto the boat and weigh the weight of the boat plus the wet soil
take the difference of the final weight ( boat and soil ) and initial weight (boat) to find the weight of the soil.
put the soil outside for 48 hours to allow the water to evaporate.
weigh the soil again.
take the difference of the weight of the wet soil and dry soil to find weight of the water in the soil ( the water that evaporated)
calculate the percent of water in the soil by using percent water= (weight of water/ weight of wet soil) x 100

Sand, Silt, Clay

take 50 ml conical vial and fill it with soil sample to the 10 ml mark
add DI water to the vial until it reaches the 30 ml mark.
add soil dispersion liquid to the vial.
shake vial vigorously for 30 seconds
let the vial rest for 48 hours.

add a small amount of sand to the pH vial
add water so that the level reaches the top of the vial
shake vial for 10 seconds and then let is rest for 120 seconds
after 120 seconds, dip 1 inch of the pH paper in the vial for 45 seconds
immediately compare the color of the strip to the scale to acquire pH

Analysis and Data:

there is no data that can yet be calculated. that will be done in pre lab calculation for the lab on 09/12/2018.

the following was collected for today

Mass of wet soil = 6.370 g

pH of soil= 6

the aseptic zone was properly maintained and there were no apparent events that may have caused contamination.

Future:

calculate % water, %sand, %silt, and % clay.

Category: Dr. Adair, Uncategorized | LEAVE A COMMENT

September 7

8/31/18 Plaque Assay of Enriched Lysate Attempt Two

Objective:

The goal of this procedure is to determine weather or not bacteria phages are present in the soil collected last week. Based on the results of the spot test it is possible that there are phages, but the previous plaque test was contaminated (see below) so now this assay will be conducted to recheck.

The overarching question this test seeks to address is: Is the presence of phage determined by species of oak tree from which soil was collected?

In other words, are specific oak tree species more likely to have Arthrobacter bacteria phages in the soil surrounding them?

The question specific to my lab table is: Is the a difference in the presence of phage between live oaks and red oaks on Baylor’s campus?

As a group we hope to expand our question to include more species as we gather data so that we can better address our overarching question.

Procedures and Protocols:

Materials:

CiDecon
70% Ethanol
Ethanol Burner
.5 ml Arthrobacter
incubator
Pipette
Test tube stand
50 ml tubes
15 ml tube
LB Broth
2X TA
1M Calcium Chloride
Agar plate
Serological pipette

In order to complete the procedure an aseptic zone was created.

Clean off the work space (lab table) with CiDecon applied with a squeeze bottle and wiped away with a paper towel
Apply 70% Ethanol with a squeeze bottle, spread with a paper towel, and allow to evaporate
Light an ethanol burner in order to use the rising heat from the flame to form the aseptic zone

Then the plaque assay on the enriched lysate was preformed.

Label two agar plates. Label one with initials, date, and description “TA control”
Label a second agar plate with initials, date, and description “Enriched Lysate Assay (HB)”
Gather the remaining enriched lysate from last procedure (found in the pipette cap seen below)
Using a Serological pipette, aseptically transfer 10 µL of the remaining enriched lysate into a 15 ml tube containing .5 ml of Arthrobacter
Reseal tube and recap pipette cap
Allow the lysate and bacteria solution to sit for 15 minutes while the agar is prepared

While the lysate and bacteria are allowed to sit in the culture tube prepare the agar

Prepare the agar according to the following recipe (makes two plates):
Under aseptic conditions, pipette 5.o ml of LB broth into a 50 ml tube. Cap the tube. *Note: This was a mistake that will be addressed later in the procedure*
Under aseptic conditions, pipette 45 µL of 1 M CaCl2 into the same 50 ml tube. Cap the tube.
Discard 50 ml tube with LB broth and CaCl2 because the measurements were wrong
Under aseptic conditions, pipette 4.o ml of LB broth into a 50 ml tube. Cap the tube.
Under aseptic conditions, pipette 45 µL of 1 M CaCl2 into the same 50 ml tube. Cap the tube.
Under aseptic conditions, pipette 5.o ml of 2X TA into the same 50 ml tube
Pipette the mixture several times to mix it

When agar preparations are finished the bacteria and lysate have been allowed to sit for 15 minutes

Pipette 4.5 ml of the contents in the 50 ml tube into the lysate and bacteria 15 ml tube
Pipette the mixture several times to mix it
Pour the mixture from the 15 ml tube into the agar plate labeled with initials, date, and description “Enriched Lysate Assay (HB)”
Cap the plate and allow the plaque assay agar to solidify for 10 minutes
Aseptically Pipette 5 ml of the contents in the 50 ml tube onto the control TA plate
Allow the TA control plate to sit for about 10 minutes before being placed into the incubator
Once the labeled plaque assay has solidified, invert the plate and place it in the incubator
Leave to incubate until next class (~94 hours)

Results:

The results of this procedure will not be immediately clear until Wednesday’s normal lab, but once they are available they will be included here.

Update: The top agar appears to have not solidified all the way because there is liquid in the plate. There do not appear to be any plaques in the areas where that agar remained intact suggesting a negative assay.

Analysis:

Because the Agar did not stay solidified it is hard to analyse these results with certainty; however, because there did not appear to be plaques on the parts of the agar that remained solid it is likely that the assay was negative. This suggests that there were not phages in the soil collected, or that there are not enough to cause results.

Future:

Due to the two unsuccessful assays, I will be collecting soil next lab in an attempt to find viable phage for isolation and purification.

Category: Lucy FIsher, Uncategorized | LEAVE A COMMENT

September 7

09/05/2018- Soil Collection for Soil B

09/05/2018

Soil Collection Sample B

Objectives:

Collect new Soil Sample
Create a research question
Collect metadata for new soil sample

Pre-lab analysis:

Analyzed the plaque assay from 08/29/2018. The plate was negative. The plates with lysate of soil sample from the same location was also negative. Conclusion, there are no bacteriophages in that soil sample.

Materials Required: Ziploc bag, sharpie for labelling, 15 ml conical vial, tool for digging and transferring soil, meter tape and electronic device for filling out the metadata survey

Procedure:

We were assigned new group members and our designated spots.
The research question was decided by the 6 people at the table. We decided to test the difference in the presence of bacteriophages in the soil of live oak and red oak.
My side of the table will be collecting a sample from a live oak.
We found our live oak right of the beach of the BSB
We collected one leaf sample from the tree ( 1 per group member)
We collected the metadata for the tree and recorded it in the survey
We collected soil in the Ziploc bags.
We then transferred 2 ml of soil to a 15ml conical
We put the leaf sample, soil sample and the 15ml conical vial in the bag.
Store the samples in the fridge to prevent change in the soil before the washing and enrichment process.

Pictures:

Analysis: no real analysis can be made for what we did today. the research question the table decided upon : ” Is there a difference in the presence of bacteriophages in the soil of two different species of oak ( live and red oak )? ”

Future notes: remember the adjustment for protocols for the washing and enrichment process. Develop better communication with peers to speed up process of decision making and planning.

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September 6

9/5/18 Soil Collection

Rationale:

In today’s lab, the goal of the class was to develop a scientific question and narrow our research field to have a method of collecting data samples. In addition to this, our own individual lab groups determined what data we would be testing and went out to our decided areas to gather soil sample.

Procedure:

Today’s lab began with analyzing the results of the plaque assays that were performed the week prior. Unfortunately, there was no phage present in the plaque assay, confirming the negative presence of phage. The data and results were recorded and the plaques were disposed of.

Plaque Assay 9/30/18

After analyzing the data, we continued with a class discussion regarding the class scientific question. Several ideas were put out into the class with the general focus being around comparing the presence of Arthrobacter-phage between tree species or comparing the phage presence between native and transplanted trees.
Our individual lab group decided to test the trees around the area in North Village due to the fire it experienced a few months prior (microorganisms have an increased presence in soil following fires), so the group walked there after the discussion ended and split the search for red and white oaks.
My group was responsible for finding a white oak, which initially was very difficult as the North Village area is predominantly Red Oak and Live Oak, but thankfully we managed to find a Burr Oak in a garden area.
The tree was surrounded by landscaping rocks and mulch, so the group had to remove the rocks and dig past the mulch to get to the soil underneath it.
While my partners dug and collected soil, I collected the metadata for the tree. First, I examined the condition of the tree and took pictures to help determine the health of the tree. After, I measured the width of the trunk 137 centimeters up the trunk, and then measured the average canopy width of the tree.
Once this was completed, the group and I used our shadows and the shadow of the tree to set up a proportion to solve for the approximate height of the tree. Once this was done, we gathered 3 leaf samples and left to turn in our soil samples.

Observations/Data/Conclusions:

Tree was surrounded by other plants that could be potential competitors for nutrients. Tree bark looked healthy, but the leaves showed spotting and slight discoloration across the tree.
Soil was incredibly wet and dark, almost like clay. Very east to mold and collect.
No nearby water source was found, but something must be contributing to the soil moisture contents.
Tree Width: 61 cm
Average Canopy Length: 225.5 cm
Burr Oak 9/5/18

Soil 9/5/18

Burr Oak Leaves 9/5/18

Next Steps:
The next steps are to clean and enrich the collected soil sample to test its presence for phage.

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September 6

9/5/18 Soil Collection

Scientific Question:

What is the difference in the amount of bacteriophage present between the soil surrounding newly planted trees versus the trees that are well established?

Rationale:

Under the premise of our newly defined scientific question, we collected soil samples from newly planted trees. We will compare the soil samples from age established oak trees to newly planted oak trees to see if bacteriophage are present in one or the other. We will eventually ascertain a lysate from this soil sample so we can test it through both spot tests and plaque assays for the presence of phage, to test our question.

Procedures:

As a class, attempted to develop a question, but then as a table, we developed our own question.
I then went out and collected a soil sample with my 15mL vial and a plastic bag so I could have extra soil. Collected the soil without touching the soil with my hands to rule out any possible contaminations from the beginning. I labeled the bag 9/5/18 ML Soil B.
I then recorded the height measurements of the tree and recorded it onto Canvas, which I will finish entering the data gathered.
Back in the lab, I transferred some of the soil in my 15mL vial into the bag since we only 2mL of soil in the vial. I then put everything into the plastic bag, my 15mL vial with 2mL of soil, soil B, and my three leaves.

Observations/Metadata:

The tree in which I gathered my soil samples were right in of the Baylor Science Building on 2nd Street. My trees height was roughly about 5m tall, and roughly 8m across. The trunk was roughly about 58cm, which indicates it is one of the new trees on campus. Observing the tree, trunk looked healthy, the leaves were green (only 3-10 leaves were brown), and the branches throughout the tree looked proportional. The tree looked healthy with no evidence of damage.

Conclusion/Next Step:

From the class conversation about our Scientific Question, we changed our initial point of view from a broad topic to a more narrow topic. We designed a question that was testable in our hands, and we went to gather soil. The sample that was collected will allow us to test if we have bacteriophage present in our soil/tree via spot tests and plaque assays. From this information, we will then be able to begin answering our many questions regarding the amount of bacteriophage present in recently planted trees versus trees that are well established.

Category: Michael Lum, Uncategorized | LEAVE A COMMENT

September 1

Plaque Assay 8/29/2018

Rationale: Perform a plaque assay to double check for bacteriophages in the soil samples

Results and Observations from 8/27/2018 spot test:

Figure 1: Contamination found on spot test plate. Results are not viable.

Figure 2: LB agar media control plate. The plate was clean and showed no signs of contamination.

Process:

Made top agar for 3 plaque assay plates (one for me, Lauren, and Lily)
The table below shows what we added to the media
1. Reagent Amount
  
  LB Broth 8.0 mL
  
  2x TA 10.0 mL
  
  1 M CaCl2 900 µL
Added 10 µL lysate to 0.5 mL artho
Let the solution stand for approximately 20 minutes
Pipetted about 1 mL of LB agar onto 1/4 of a shared controlled plate
Separated the remaining agar into 3 separate 50 mL tubes for each lab partner (about 6 mL in each)
Added artho + lysate solution to my tube
Poured entire contents onto sterile plate (that already had LB agar on it)

Category: Rachel Melone, Uncategorized | LEAVE A COMMENT

September 1

Testing Question 8/29/2018

Rationale: Create an experimental question as a class

Are there different bacteriophages in the soil of red trees versus white trees on the Baylor Campus?

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BEARS in the SEA 2018-2019

A digital lab notebook for phage research

09/12/2018- Soil Metadata analysis and Spot test

9/12/18 Spot Test/Plaque Assay/Continuation of Soil Metadata

9/10/18 Soil Washing and Enrichment and Metadata Soil B

09/10/2018- Washing And Enrichment of Soil B

8/31/18 Plaque Assay of Enriched Lysate Attempt Two

8/31/18 Plaque Assay of Enriched Lysate Attempt Two

Objective:

Procedures and Protocols:

Results:

Analysis:

Future:

09/05/2018- Soil Collection for Soil B

9/5/18 Soil Collection

9/5/18 Soil Collection

Plaque Assay 8/29/2018

Testing Question 8/29/2018

Reagent	Amount
LB Broth	8.0 mL
2x TA	10.0 mL
1 M CaCl2	900 µL