Description of procedure (what you actually did, NOT what you were told to do)-storage
Observations/Results/Data
AUGUST 22, 2018
CLEANSING AND SEPARATION OF OAK TREE DIRT
OBJECTIVE: To clean and separate out the soil sample, into a direct and enriched sample
PROCEDURE:
The tables were sanitized and lamps were lit to create an aseptic environment
A 50mL test tube was filled to the 15mL with soil that was collected 48 hours prior to the lab in section 8, from a healthy oak tree, seen in figure 1
Then LB broth was added to the tube util it reached the 35mL mark
The tube was then shaken by hand and vortex for 15 minutes
The tubes were then taken to the centrifuge when they centrifuged as a class
The contents of the soil had separated out into supernatant in the top and a solid in the bottom
The supernatant was then pipetted into a filter where pressure was applied to it to force it through the .22 𝝁m and dripped into a 50mL tube as seen in figure 2 (must be conducted in fume hood)
The supernatant was filtered until 10mL was filtered out
The 10mL of the filtered supernatant had .5mL of Arthro added to it (This became the enriched sample), and the sample was then placed in the fridge
The excess unfiltered supernatant was put into a separate test tube and placed in the fridge
NEXT STEPS: Conduct Spot test with samples + filter out direct sample
