April
26
APRIL 24TH- LABS
- APRIL 24TH 2019
- OBJECTIVE:
- Start to work on and create the presentation, and finalize abstract
- PROCEDURE:
- A Google slides file was hared with all group members, in which information was added to the slides
- Comments from the rough draft of the abstract were used to edit. And create a new abstract using google docs
- RESULT:
- A google slides presentation was created and edited
- A final abstract was created:
- Bacteriophage genomic analysis can demonstrate the diversifying effects of mosaicism across phages through the presence of conserved domain gene groups. One mechanism that supports genetic diversity among bacteriophages is Horizontal Gene Transfer, which can allow transmission of localized cassettes. A certain class of cassette involved in DNA replication gene function of the viral genome was identified in NapoleonB. The DNA replication module was noticed to have a specific ordering in common with certain other bacteriophages outside of it’s cluster. The online tool Phamerator was used to visually represent genomes to compare the presence and ordering of genes belonging to a similar module. Genomes of phages that infected multiple different actinobacteria were all found to contain a common gene cassette. A protein folding tool RaptorX was used in tandem with Jmol, a tool for visualizing predicted protein structures, to visualize the function and similarity among these common genes. Clustal Omega and MEGA are sequence alignment tools that were used to make phylogenetic trees. The use of Splitstree allowed for the construction of a phylogenetic network to visualize the parsimony amongst the different bacteriophages studied. The data collected has shown that members of certain clusters can share synteny with members outside of their own cluster. The commonality among the ordering and presence of genes in these clusters provides evidence that allows for the prediction of the function of genes which had no defined predicted function before. Furthermore, if future phages are discovered with this replication cassette, there will be more evidence for making gene function calls using synteny with phages outside of just it’s own cluster.
- CONCLUSION:
- Presentation was created
- Abstract was edited and finished
- FUTURE STEPS:
- Continue work on presentations