Rationale:

The purpose of today’s lab was to rewrite the final abstract as well as begin the final presentation. Also Jmol was used to try and represent endolysin complex with Glycine-Glycine bridges

Tools:

• Jmol
• Microsoft Word
• Microsoft Powerpoint

Procedure:

• Final abstract was rewritten using the notes left on the previous abstract submission.
• A general outline of the final presentation was made, with general ideas for slides put out onto each slide.
• Used Jmol to visually represent Glycine-Glycine fitting into the catalytic region of our M23 peptidase endolysin.

Results: 

Copy of the Final Abstract

Visual Representation of Glycine-Glycine bridges fitting in active site.

Conclusions:

With the final abstract written, we are able to continue with creating the presentation for the CURES Symposium. Also, the ability to represent Glycine bridges fitting into NapoleonB’s endolysin catalytic region gives us another method of comparison between M23 Peptidase and PGRP endolysins.

Next Steps:

Our next steps for this project are to finish the final presentation for the symposium as well as finalize the PGRP catalytic region.