4/15/19

Rational:

To compare the hydropathy plots of possible holin genes in other Arthrobacter phages. To create the outline for the researh presentation.

Procedure:

• Took the amino acid sequences of the predicted holin genes of AM cluster phages and two outside the cluster
• Created hydropathy plots using TMHMM with these sequences
• Added the project title and guiding question to the outline
• Created a basic outline for the abstract

Observations:

• The hydopathy plots of the AM phages were very similar to NapoleonB and at first glance some look almost identical

Fig.15 – NapoleonB’s hydropathy plot (left) looks almost identcal to Arcadia’s (right) another AM cluster phage

• The differences between NapoleonB’s hydropathy plot and phages from other clusters were more obvious than with phages from the AM cluster

Fig.16 – NapoleonB’s hydropathy plot (left) shows more differences with Madamato (right) a BI cluster phage

Conclusion:

Created hydropathy plots for all of the AM phage predicted holins and a few others to compare. Finishd the outline for the research project presentation. Next lab we will write the abstact for our research project.