Rationale:

To continue working on our IRP, by further looking at background research, protein structure, and multiple sequence alignments.

Procedure:

1. The structure of gene 93 was looked at on TMHMM, in roder to provide evidence that it is a transmembrane transporter protein.
2. MSA’s were run in order to show phylogenetic relationships.
3. Further research was done into the pham changes.

Results/Data:

According to TMHMM gene 93 is indeed a transmembrane protein. The pham we were looking at on Monday was split, eliminating a lot of the confusion that was present on Monday. The new MSAs conducted show much more similarity between the different phages/bacteria.

Conclusions/Next Steps:

We can conclude that gene 93 is an NMT. We can also conclude that there was an apparent error in the pham system, as it is now fixed and makes a lot more sense. We can also start creating a phylogenetic tree based on our MSA data. The next step will be to continue investigating NMTs and NMN, along with conducting more MSA’s on more phages and bacteria in order to increase our understanding of the phylogenetic relationships and origins of the NMT gene in NapoleonB.