April
11
DNA Day 20
8 April 2019 ✷ Independent Research
Rationale: Groups began work on their projects. The genome of NapoleonB was scrutinized to answer the question on the relationship between its genes that were AM-phage-specific and genes that were present in non-AM phages and percent CG.
Procedure
- The new annotations (including inserted genes) was added into the DNA Master file for NapoleonB and the percent GC and GC3 of each gene was calculated by DNA master and recorded by group members.
- The genes were sorted by whether or not they were specific to AM and then averages and statistical analysis was performed by Lucy.
- This procedure was repeated for a phage from a different cluster.
- The average %GC for the entire genome (rather than by each gene) for all the clusters was provided by PhagesDB and statistical analysis was performed with this information as well.
Results
Pictured is the data recorded today.
Conclusion
AM phages have the lowest % GC of all the clusters. The genes that are AM specific seem to have a lower percent GC than those genes that exist in other clusters.
Future plans
Find more data!