Rationale

Find resources through primary literature, form and revise guiding question, and start making results and analyze them.

Procedure

1. found 5 sources and revised question
2. NCBI blasted NapoleonB’s gene 93 sequence
3. Found 6 bacteria and 6 non AM (3 AU and 3 EG) clusters that HHPred called as NMT
4. multiple sequence alignment with all 18 sequences, 6 AM, 6 non AM, 6 bacteria, 3 AU, and 3 EG.
5. Saved pdf filed for all 6 multiple sequence alignment results

Conclusion/Next Steps

We found that when we blasted NapoleonB, we found that it had some hits from bacteria. So that raised a hypothesis that NMT from NapoleonB might have come from or evolved from bacteria. From the MSA results from all 18 sequences, we found that bacteria and AM/AU cluster had more similarity and the EG cluster was pretty different compared to everything else. At one point, we found that there might have been a mistake in the pham results that some were called as a nicotinamide riboside transporter/transferase. The next day, our phage were put into a different pham from that other clusters. We doubled checked through raptorx and TMHMM that our phage’s sequence is indeed a NMT. In the next time, my group and I will make a phylogenetic tree and a figure to show which part of the sequences were similar from which cluster/bacteria