April
12
4/10 Lab Journal
Purpose: Continue Individual Research
Procedure:
- Searched for Phages containing an NMT in their genome to compare to the NMT found in NapoleonB and other AM cluster phages
- Found three AU cluster phages for future research
- Found three EG cluster phages with NMT genes
- Added all the fasta files for the NMT genes from the new phages, AM cluster, and bacteria to a splitstree diagram
- Fought splitstree for almost a half hour to get it to work
- Eventually manipulated the program to do its job
- Found that with the present data we couldn’t put together a map to truly represent the genealogy of the gene
Results: Found that the splitstree graph will be harder to create than previously imagined. Will need more samples.
Future Plans: Continue finding more NMT fasta files to make into a coherent phylogenetic tree