Title: Comparing Base Pair Repeats

Date: 3 April 2019

Rationale:  To begin the major research project, the group will be examining a 51-base pair repeated sequence in NapoleonB’s genome.

Tools: 

• DNA Master
• Gepard Alignment
• PhagesDB
• NCBI BLASTn

Procedure: The 51 bp sequence was BLAST’ed and the results compared using a phamerator map and DNA Master annotated FastA files to investigate the nature of the repeated sequence.

Results/Observations: It was observed that the 51 bp sequence repeated around 25,500 base pairs in the AM cluster after an NKF gene. However, the repeat was found after a minor tail protein in NapoleonB.

Conclusions/Next Steps: The sequence will continually be investigated to find if any more evidence can be found (perhaps in the predicted protein structure or conserved domain) to find out if the sequence has any meaning or if the NKF call can be reversed.