Purpose: Investigate what affects the presence of multiple plaque morphologies in NapoleonB and other AM Cluster Phages

Procedure:

• Downloaded Fasta files for all AM Phages
• searched for phylogenetic tree generators
• Settled on program called splitstree
• Plugged in Fasta Files
• Generated Phylogenetic tree

• Determined the tree was not populated with enough data to find any correlation between phage evolution and the presence of multiple morphologies
• Revised question to begin research into Nicotinamide Mononucleotide transporters, specifically the one coded for in NapoleonB

Results: We have a revised question and a new simple procedure to find our nicotinamide mononucleotide transporter (NMT)  in other phages, to determine its origin.

Future Plans: Investigate the NMT in NapoleonB and associate it with other phages with similar proteins or nucleotide sequences.