Rationale: We are beginning to look in to the question and building a plan for moving forward with looking into this research question.

Tools: NCBI Blastp, Word, Phamerator

Procedure:

1. Explored more into the different clusters that contain the pham 19129, and found not all of the clusters infect arthrobacter.

2. Refined and reworded the research question.

3. Blasted the amino acid sequence for the DNA polymerase in NapoleonB’s genome against actinobacteria. Found it hit to Streptomyces.

4. Made a plan for conducting the research going forward.

Results:

Blast results for the DNA polymerase in NapoleonB’s genome against actinobacteria.

Conclusions and Future Work: Now that we can see some sort of start point with the streptomyces the plan is to analyze the differences between the hosts and blast the genes we found similarities in against the different hosts. From there we can compare the slight differences in amino acid sequence and nucleotide sequence in the cassette that contains the three shared genes.