In the Literature (3/27/19)
Rationale:
Create a procedure to perform to test research question.
Procedure:
- Brainstormed procedure in groups.
- Performed NCBI BLASTp.
- Started reading in the literature about the mechanisms of holin and endolysin.
Results:
The following images below show the NCBI BLASTp.
The following articles were of interest.
https://pubs.acs.org/doi/10.1021/acschembio.5b00875
https://onlinelibrary.wiley.com/doi/full/10.1111/mmi.13448
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116005/
https://www.sciencedirect.com/science/article/pii/S0065352718300563?via%3Dihub
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012431/
Conclusion:
The NCBI BLASTp revealed that both the holin and endolysin genes shared CDD meaning that enough similarity exists between these genes among phages for it to be a legimate research question. Also, many articles found on holin and endolysin, showed the research question purposed was legitimate.
Future Work:
Further research into the mechanisms of holin and endolysin will be performed to achieve a thorough understanding before starting protein analysis.
