February
18
Checking LORF of Gene 50 2/18/19
Rationale: There were some calls that were a bit difficult so as a team we revised certain genes assigned to us.
Tools: DNAMaster, NCBI, Phamerator, GeneMark, PhagesDB
Procedure:
- Looked at gene 50’s reading frame to determine if I should elongate it to the LORF.
- Checked starterator, blasted both lengths of the gene, and checked the Coding Potential coverage to determine I shouldn’t bring it back.
- Also the better RBS score supported my call.
Results:
Conclusions and Future Work: The call was correct, there is no need to move the gene to its LORF. Next we will do a group-wide analysis of the entire annotation to make sure we don’t see any glaring issues. We will also begin the process of making a presentation of our findings.