Rationale:

To begin the genome analysis of phage NapoleonB, DNA Master has been chosen to be the software to annotate the genetic sequence. PhageNotes is also introduced as a convenient way to annotate genes and revising them. Abstract for the presentation was discussed, since an abstract should concisely inform readers or poster viewers the important points of the research.

Materials:

• Laptop
• DNA Mastering Program
• Gene sequence of phage NapoleonB

Procedure:

1. The whole annotation result was reviewed as a class and few adjustments were made, mostly data input error.
2. The important points that should show up in an abstract and a poster was discussed.

Results/Next Steps:

There are a couple large gaps in the NapoleonB genome that is conserved but showed no coding potential and protein blast hits, and a reverse blast hit was found in the forward gene region.

In the next lab abstracts and posters would be the main focus.