Title: NapoleonB Annotation

Date: 11 February 2019

Rationale: The annotation for NapoleonB has begun and the genome will be sequenced as thoroughly as possible in order to determine function in relation to other similar phages

Tools: 

• Microsoft Surface Pro 5 Tablet
• DNA Master Software
• NCBI BLASTp tool
• HHPred tool
• GeneMark
• PhagesDB BLAST
• Phamerator

Procedure: Using the above tools, genes 45-48 were annotated completely (with the exception of starterator “ST”). Gene 46 was deleted due to convincing evidence of a bad call. For each gene:

• A start codon call was made based on the Glimmer/GeneMark calls or lack thereof
• The product sequence was BLAST’ed to determine any present function
• A function call was made and any supporting evidence from PhagesDB, Phamerator, and HHPred was logged

Results/Observations: The following picture contains the results of the annotations:

Conclusions/Next Steps: The starterator annotations need to be completed along with gene 95’s complete annotation. All but one gene was labelled as “NKF,” the gene that was able to be identified was an HTH DNA binding domain protein. Once the remaining genes are annotated, NapoleonB will have a completed annotation ready for analyzing.