Rationale:

To begin the genome analysis of phage NapoleonB, DNA Master has been chosen to be the software to annotate the genetic sequence. PhageNotes is also introduced as a convenient way to annotate genes and revising them.

Materials:

• Laptop
• DNA Mastering Program
• Gene sequence of phage NapoleonB

Procedure:

1. The gene that was called on Phagesdb but not DNA Master was found, and protein sequence was Blasted on NCBI pBlast, Phagesdb pBlast, HHpred, NCBI CDD.
2. Questions about annotations of gene 77-80 & *99 were submitted on canvas to lab TA’s.

Results/Next Steps:

The gene was found to be a match with Arthrobacter phage Circum on NCBI pBlast, however, the functionality of the protein is unknown, since the match was with a hypothetical protein. There were no good hits on HHpred, and no conserved domain was detected on CDD.

The next lab would be writing an Abstract and learning about Poster Design.