Rationale:

Genes from NapoleonB were assigned and the rest of the lab was given to annotate the four assigned genes.

Tools:

• Personal Computational Device
• DNA Master
• NCBI
• PhagesDB
• GeneMark

Results:

• After the genes were assigned, gene 33 was viewed on DNA Master to determine if the opening reading frame had the most coding potential if the second biggest opening reading frame had the most coding potential.
• To determine this, the base pairs for the second opening reading frame was changed and viewed on GeneMark and compared with the coding potential of the original open reading frame. It was concluded that the coding potential for the original open reading frame was best because it covered all the coding potential

• The opening reading frame was reverted back to its original frame and the product was used to compare the sequence with other sequences in databases such as NCBI, HHPred, and PhagesDB.
• With these databases, other parts of the annotations were determined in order to form the complete annotation.
• The annotation for gene 33 was

Conclusion:

Upon the assignment of the genes of 33-36, the annotation of gene 33 began. Because there was an overlap between gene 32 and 33, the open reading frame was shifted to the second best; however, it was determined that the original reading frame was the best because it covered all the coding potential, unlike the second reading frame. After the best reading frame was determined, the different parts of the annotation were found from databases such as NCBI, GeneMark, HHPred, PhagesDB, and DNA Master. The function of gene 33 was found to have no known function by NCBI, HHPred, and PhagesDB. The annotation for gene 33 was completed.

Future:

In the future (2/13/19), genes 34-36 are to be annotated and annotation of gene 33 is to be checked.