February
12
DNA Day 7
11 February 2019 ✷ NapoleonB DNA Annotation!
Rationale: NapoleonB’s genes were assigned to each student to annotate and record in order to complete the genome annotation for the phage. Annotations for genes 21-24 and 97 were started and the annotation for gene 21 was completed.
Procedure
- PhagesDB was opened and the FastA file for NapoleonB was downloaded
- DNA master was opened and NapoleonB was auto-annotated
- PhageNotes was opened and each aspect of the DNA annotations for genes 21-24 was entered into the program, however, only 21 was annotated to completion and 22 was nearly completed.
- Blasts for products (protein) were run on PhagesDB, HHPred, and NCBI to ensure accurate and up-to-date blast information.
- Phamerator was opened for AM phages and the synteny of NapoleonB was analyzed compared to genomes of phages in the AM group.
- From PhagesDB, starterator for each gene was opened and compared (this was only done for genes 21 and 22 so far) and both agreed with starterator.
- The information was recorded and saved in the google sheet.
Results
the complete annotation for gene 21 and the partial annotation for gene 22, as entered into PhageNotes, are as follows:
Conclusion
There was some confusion regarding the function of gene 22 of NapoleonB, and time was limited so the annotation wasn’t completed. Further research needs to be completed in order to make an informed decision on the final function of gene 22 because the databases do not agree on the function–PhagesDB and NCBI say it aligns with a tail protein, but HHPred and the conserved domain information both suggest an unknown function related to botox.
Future plans
The function of gene 22 will be further analyzed and researched and the annotation will be finalized. Then, genes 23, 24, and 97 will be annotated.