Rationale: Begin annotating assigned genes of NapoleonB

Process: Used DNA Master, GeneMark, NCBi Blast, NCBi cd, phagesdb, and HHPRED to annotate gene 81 of NapoleonB

• covered all of coding potential and more so I did not change the start codon
• had an overlap of 6bp
• evidence that gene 81 could code for a helicase

Result:

SSC:46764 – 48074, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Arthrobacter Phage Circum gp80 NCBI BLAST q1:s1 0.99 0, Aligns with Arthrobacter Phage Circum gp80 PhagesDB BLAST q1:s1 0.99 0, Gap:6bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 1.808 -5.07 No, F:Helicase, SIF-BLAST:ATP-dependent helicase Supported by NCBI BLAST Arthrobacter Phage Circum gp80 ALY08763.1 0.99 0, ATP-dependent helicase Supported by PhagesDB BLAST Circum gp80 KU160642 0.98 0, , SIF-HHPred:Predicted DNA helicase supported by KOG gpNA KOG1016 0.3166 , SIF-Syn:NKF

Next Steps: continue annotating NapoleonB genes 82-84