Further Annotation of Phage Elesar Genes 12 and 13

Date: 2-4-19

• Rational
  • The rational for this lab was to annotate the function data for two genes from phage Elesar in order to gain practice in annotation before transitioning to phage NapoleonB.
• Procedure
  1. DNA Master was opened.
  2. File > Open > Archived DNA Master File was used to open the same Elesar FastA file from before.
  3. Gene 12 was selected through the listing of predicted genes.
  4. The annotation notes were analyzed and copied to the PhageNotes Google Sheets page.
  5. Gene 13 was selected through the listing of predicted genes.
  6. The annotation notes were analyzed and copied to the PhageNotes Google Sheets page.
  7. Using the acquired information, the 12th and 13th genes were annotated.
• Results
  • The following annotations were copied for Elesar gene 12 :
    • SSC:8224 – 8568, CP:No There is an overlap with the previous gene, SCS:Both, ST:SS, BLAST-Start:Aligns with Nandita gp13 NCBI BLAST q12:s1 0.87 5E-58, Aligns with Phage Ryan gp13 PhagesDB BLAST q12:s2 0.87 1E-48, Gap:7bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 2.463 -3.67 No, F:head-to-tail stopper, SIF-BLAST:head-to-tail stopper Supported by NCBI BLAST Nandita gp13 AYN58635.1 0.87 5E-58, head-to-tail stopper Supported by PhagesDB BLAST Ryan gp13 MH834627 0.87 1E-48, SIF-HHPred:NKF, SIF-Syn:head-to-tail stopper, upstream gene is head-to-tail adaptor, downstream gene is NKF, just like in phage Ryan
  • The following annotations were copied for Elesar gene 13 :
    • SSC:8565 – 8840, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Nandita gp14 NCBI BLAST q13:s1 0.76 3E-38, Aligns with Phage Nandita gp14 PhagesDB BLAST q13:s2 0.76 7E-31, Gap:4bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 1.137 -7.054 No, F:NKF, SIF-BLAST:NKF Supported by NCBI BLAST gp, SIF-HHPred:NKF, SIF-Syn:NKF
• Conclusion
  • All the genes present in Phage Elesar have been copied to a single document in order to share the data.
• Future Plans
  • The next step is to continue annotating more genes on Elesar for practice before transitioning to NapoleonB.