2/6/19

Rational:

To enter the annotations made on genes 20 and 21 for Elesar on PhageNotes in order to share it with the rest of the class. This makes it easier and faster to annotate the entire genome of Elesar. Also to check the annotations of two other genes.

Procedure:

• Entered my name next to the genes I annotated in PhageNotes and added the auto-annotation calls
• Entered the information for SSC, CP, SCS, LO, BLAST-Start (NBCI), Gap, F, SIF-BLAST (NBCI), SIF-HHPRED, and SIF-Syn
• BLASTed genes 20 and 21 in phagesdb and entered the needed information
• For RBS entered only the SD final score, whether it was the best, and the z-score
• Used the BLAST for phagesdb to predict the function of the genes
• Checked genes 8 and 9 by annotating them myself and comparing my annotations with the annotation entered

Observations:

• Information for BLAST-Start and SIF-BLAST using phagesdb was needed
• For gene 9 the start codon was moved back, but the SCS did not support this change
• The rest of the annotations for genes 8 and 9 seemed correct

Fig.2 – This image shows the information found on gene 9 from phagesdb. The information matches the annotations entered in PhageNotes.

Conclusion:

The annotations for 20 and 21 were finalized and entered into PhageNotes. Genes 8 and 9 were also checked for mistakes in the annotations. Next lab I will start annotating NapoleonB along with the rest of the class.