February
8
Entering Elesar Gene 44 and 45 into Phage Notes (2/6/19)
Rationale:
The purpose of the lab was to put Elesar’s Gene 44 and 45 annotation into Phage Notes and to learn more about Phamerator and tRNA’s.
Tools:
- DNA Master
- NCBI Blast
- HHPred
- Phage Notes
- PhagesDB – Blast
Procedure:
- An account for Phamerator was created.
- The file, which contained annotations of Gene 44 and 45, was opened and annotations were added into Phage Notes.
- NCBI Blast, HHPred, and PhagesDB were referenced to confirm that annotations were up to date.
- Annotations of Gene 50 and 51 were verified for accurate annotations.
Results:
Gene 44 and 45 Annotations :
- PhagesDB showed that a better match was found for Elesar Gene 44, and therefore changes were made to the annotation.
Conclusion:
More knowledge on annotations was obtained from practice on Gene 44 and 45, and revision of Gene 50 and 51. For annotations to be finished, SIF- synteny and Starterator have to be completed. Skills and knowledge acquired from annotations of Elesar Genes will be used for the annotation of Napoleon B.
Future Work:
In the future labs, SIF-Synteny and Starterator will be completed and the process to annotate Napolean B will be started.