Rationale:

The purpose of today’s lab was to understand how to annotate supporting information of function (SIF) using a variety of databases such as NCBI and HHPred. The information that was given off each database was explained and taught how to use the information to create an annotation. Towards the end, the previous annotation was expanded upon for practice with the addition of SIF-BLAST (NCBI and HHPred.)

Tools:

• Personal Computational Device
• DNA Master
• NCBI
• HHPred

Results:

• HHPred was introduced and was used to demonstrate how to use the information for annotations for evidence.
• NCBI was then used to annotate for SIF-BLAST (NCBI)
• After the annotation, a practice was done on the previous annotations that were assigned (Gene 46 and 47)

Conclusions:

After the introduction of HHPred which provides evidence of a match between two different sequences, NCBI was then used for the same purpose. After the lesson, some practice was done with the assigned genes 46 and 47.

Future Plans:

Other databases will be shown to provide further evidence of the match between the sequences.