Rationale:

To fix the annotations of Elesar genes 48 and 49 and add SIF-BLAST and SIF-HHPred information.

Procedure:

1. Using a previously open and auto annotated copy Elesar on DNA Master genes 48 and 49 were viewed.
2. Fixed coding potential on gene 48 and added ran SIF-BLAST and SIF-HHPred for both.

Results:

Gene 48:

SSC: 34620, 35063 CP: yes SCS: Both BLAST-Start: Elesar, gp45, PhagesDB, Query 1 to Subject 1, 100%, 6e-83 Gap: 113bp gap LO: yes RBS: Kibler7, Karlin Medium, 2.611, -3.929, no SIF-BLAST: NKF SIF-HHPred: NKF

Gene 49:

SSC: 35215, 36054 CP: yes SCS: Both BLAST-Start: Nandita, NCBI, Query 1 to Subject 1, 95%, 8e-165 Gap: 151bp gap LO: yes RBS: Kibler7, Karlin Medium, 2.970, -3.344, yes SIF-BLAST: HTH DNA binding protein, NCBI, Nandita gp49, AYN58672.1, 82%, 8e-165 SIF-HHPred: Transcription factor MBF1 [Transcription], KOG, KOG3398, 98.31%, 5.6e-9

Conclusions:

Based upon the data gene 49 can be reliably called to be a gene that codes for Helix turn Helix DNA Binding Protein. For gene 48 however, there is no known function for its product as agreed to by the various data sources.

Next Steps:

The next step will be to continue practicing the annotation of genes and to familiarize myself with DNA Master with the future goal being the complete annotation of the phage NapoleonB’s genome.