Rationale

Re-annotation and corrections were made in the lab as well as peer review for other gene annotations. This helped us learned our mistakes and help correct others to prepare us for future annotation of NapleonB

Procedures

• Opened DNA master with Elesar file
• Blasted gene 4 and 5 through NCBI, phagesdb, and HHPred
• Used annotation file to see any mistakes and made correction to template
• Repeated with genes 20 and 21 for peer review

Observations

Gene 4:

Original Glimmer call @bp 1349 has strength 7.44

SSC:1349,1984 CP:yes SCS:both ST: BLAST-Start:Nandita,5,phagedb,query 1 to subject 1,95%,3e-122 Gap:0 LO:NA RBS:Kibler7,Karlin Medium,2.230,-4.085,no F: SIF-BLAST:terminase small subunit,phagesdb,Nandita_5,AYN58627,95%,-122 SIF-HHPred:teminase small subunit,COG,COG3747,75%,99.57 SIF-Syn:

Gene 5:

Original Glimmer call @bp 1971 has strength 13.74

SSC:1971,3668 CP:yes SCS:both ST: BLAST-Start:Nandita,6,phagedb,query 4 to subject 5,99%,0 Gap:14 LO:NA RBS:Kibler7,Karlin Medium,1.724,-5.684,no F: SIF-BLAST:terminase large subunit,phagesdb,Nandita_6,AYN58628,97%,0 SIF-HHPred:terminase large subunit,Terminase_1,PF03354,87%,100 SIF-Syn

Results

Wrote out the e value incorrectly compared to last lab day. The e value changed for gene 4 according to NCBI. Blast results from multiple sites highly suggested that both genes were a terminase.

Next Steps

Continue to re-annotate until no mistakes are made and use those skills to apply to NapoleonB