Rationale: DNA from Elesar genes 32 and 41 were annotated, with the addition of the protein function for practice in preparation for the annotation of NapoleonB.

Procedure: 

• FASTa file for Elesar was opened and auto-annotated
• The “features” tab was opened and the “note” box was filled with the annotations for gene 32 and 41.
• Errors from the previous lab were corrected. Gene 31 LO was corrected and gene 42 start codon was pushed back and LO was fixed.
• The SIF-BLAST and SIF-HHPred commands were annotated
• The product codes were run through NCBI Blast and HHPred (+COG and PFam databases) and that information was added to the annotation as indicated by NCBI.

Results:

Gene 41

SSC: 30932 31219 CP: yes SCS: Both Blast-Start: Mycobacterium virus Brujita, 35, NCBI, Q7: S2, 60%, 4e-13 Gap: 607 LO: no RBS: Kibler 7, Karlin Medium, 0.897, -6.997, no F: This family of presumed lipases and related enzymes are similar to pfam00657

SIF-BLAST: This family of presumed lipases and related enzymes are similar to pfam00657, NCBI Protein, hypothetical protein PBI_BRIDGETTE 30, AYN57297.1, 81%, 0.0

SIF-HHPred: General function prediction only, KOG, Putative Zn-finger protein, KOG1777, 65%

Gene 32

SSC: 23223 26093 CP: yes SCS: Both Blast-Start: hypothetical protein PBI_BRIDGETTE, 30, NCBI, Q601: S62, 81%, 0.0 Gap: 7 LO: no RBS: Kibler 7, Karlin Medium, 2.117, -4.852, no F: DNA-binding helix-turn-helix domain. SIF-BLAST: DNA-binding helix-turn-helix domain, NCBI Protein, HTH 17, pfam12728, 59%, i.88e-09 SIF-HHPred: Pyocin activator protein, PF, ProcinActivator, PF11112.8, 76%

Conclusions/future plans: Annotations help analyze the function of a gene and is useful in research since it allows you to compare the genes with different species that potentially contain the same gene. Determining the function of genes based on other’s research and the known data of the genes will help speed the process of annotation and learning more about the species genome. Become familiar with the new database HHpreb and understand what the results mean. This same method will be applied to NapoleonB in order to analyze its genome.