Rationale: Learn how to annotate specifically the functionss and SIF-BLAST, SIF-HHPred, and SIF-Syn

Materials:

• Computer
• DNA Mastering Application
• Annotation Key
• HHPred
• PhageDb
• NCBI BLAST

Procedure:

• Booted up DNA mastering and revised QTM 5
• Added annotations to gene 24 and 61 (Assigned last class)
  • Specifically annotated SIF-BLAST, SIF-HHPred, and SIF-Syn
• Utilized SIF-HHPred to predict protein structure and shape
• Uploaded annotations into spreadsheet

Observations:

The hits of the predictions. The first hit has the lowest evalue, which indicates a probable match to the query gene

The prediction of gene 61

The names of hits off the database which can be used to determine the function of the gene

The prediction of gene 24

Next Steps/Conclusion:

After this lab, the sequencing of NapoleonB may start. These previous labs have proven as practice for annotating, and will show when annotating of NapoleonB begins.