1/28/19

Rational:

To learn what information to put down and where to find it in order to annotate individual genes.

Procedure:

• Opened the auto-annotation for Elesar
• Put the start of gene one back in order to cover the coding potential and have the longest ORF
• Wrote the start and stop coordinates for gene one
• Used the GeneMark analysis of Elesar to determine whether the current start of the gene covers the coding potential
• Wrote who the start codon was called by as well as who it was selected by
• BLASTed the protein code for gene one to look for significant alignments with other genes

Observations:

• The start codon was called by both GeneMark and Glimmer, but called by me
• The BLAST results showed no significant BLAST alignments
• Under the new start all of the coding potential was covered

Conclusion:

The annotations for SSC (start/stop coordinates), CP (coding potential), SCS (start choice source), and BLAST-start were determined and written in the template in the notes section for the gene. Next lab I will learn how to do the rest of the annotations and continue to annotate gene 1 and other genes.