Rationale: Continuing to practice annotating I will be annotating genes 28 and 29 of Elesar by myself.

Tools: NCBI, PhagesDB, DNAMaster

Procedure:

1. Opened the saved file from the previous lab, which had already be auto-annotated and had the template loaded.
2. Recorded the SSC, CP, SCS, GAP, and LO for gene 28.
3. Blasted the product of the gene using both the PhagesDB and NCBI databases. Recorded results under Blast-Start.
4. Highlighted ORF in the frame viewer and used a Kibler7 Scoring Matrix and a Karlin Medium Spacing Matrix to find the Z score and Final Score.
5. Repeated steps 2-4 for gene 29.
6. Posted and saved file.

Results:

Blast results for Gene 28

Blast Results for Gene 29  (Blast results were the same for NCBI and PhagesDB so only NCBI shown)

Conclusions and Future Work: I was able to succeed in annotating the gene by myself. I noticed the importance of blasting using both databases as they could have different results such as in the case of Gene 28. In the future I will continue working on annotating Elesar as well as filling out the rest of the required parts of the annotation.