Rationale

Today we will finish annotating Gene 1 of Elesar and will independently annotate other genes in the genome.

Procedure

• The work done on the annotation of Gene 1 was completed by re-opening the FASTA file and annotations.
• Genes 42 and 43 were assigned to annotate for practice, and the following categories were completed with respect to the genes assigned: SSC:, CP:, SCS:, ST:,BLAST-Start:, Gap: LO:, RBS:

Results

Blast results are shown above for Gene 42 and 43. The results show the NCBI database and the matching results for each query.

Gene 42: SSC: 31255, 31935, CP: yes, SCS: both-cs, BLAST-Start: [hypothetical protein PBI_MAJA_36, 36, NCBI, 46, 2, 2e-46], Gap: 3 overlap, LO: N/A, RBS: [Kibler 7, Karlin Medium, 2.325, -3.883, no]

Gene 43: SSC: 31932, 32198, CP: yes, SCS: Glimmer, BLAST-Start: [hypothetical protein PBI_RYAN_46, 46, NCBI, 1, 1, 2e-53], Gap: 3 overlap, LO: N/A, RBS: [Kibler 7, Karlin Medium, 2.214, -4.470, no]

Conclusion/Next Steps

The practice of annotating the genes can be beneficial for future use. Annotations will occur with NapoleonB and it is imperative that an understanding DNA Master is possessed.