Rationale

Annotate and update template for genes 4 and 5 from elesar. Practice learning how to annotate each gene.

Procedure

1. Opened DNA master with elesar fasta file
2. Opened DNA frames, clicked on ORFs, highlighted target gene, clicked on RBS
3. Blasted gene 4 via phagedb
4. Filled out template for SSC, CP, SCS, Blast-start, Gap, LO, and RBS and used guidelines and annotation key for assistance
5. Repeated same procedures above for gene 5.
6. Clicked post after each gene annotation and saved as new dnam5 file.

Observations

Both gene 4’s and 5’s scores are not the lowest, which is why RBS has “no” on both of them, but there is still a high chance that both genes coded for Nandita.

Next Steps

Finish the rest of the template and have a better understanding of annotating any gene from elesar to prepare the annotation of NapoleonB.