Purpose and Rationale: Annotate Phage Elesar to find the proper starts, stops, and other features to the genes. Annotating these genes will help us refine the process of annotation and improve mastery of the computer program.

Procedure:

• Opened Elesar dnam5 file.
• Continued adding annotation notes to gene 1.
• Assigned genes 26, 27 to complete annotations on.
• Found start/stop of gene 26
• Determined that the start and stops matched the coding potential found in Genemark.

Elesar Gene 26, showing the beginning of coding potential at the same base as the start codon, 18815, and stopping pair 19690.

• Blasted gene in both databases. Found to be most similar to a bacterial peptidase, known as M23.

This particular peptidase is found in Arthrobacter sp. KI72, potentially raising a “chicken or the egg” question

• Continued to fill in the description notes with the remaining information

Began work on gene 27

• Found the auto-annotated start and stop, and that they were accurate with the coding potential and the stop codon.
• Coding potential continued after the end of gene 27, into gene 28, however, the stop codon provides the end of the gene.

Note the gap just after base pair 20,000, where the coding potential dips but does not hit zero. It flows right into gene 28.

• Blasted the Gene, and found it to be similar to bacterial proteins without a known function.

• Finished writing genetic notes into the description

Results: Completed annotating all portions of Genes 26 and 27 that had been taught to us. There are other portions of the notes left to be completed, but that will have to wait until we are instructed on what to do.

Future Plans: Continue Annotations as instructed next lab period.