Rationale: Practice BLASTING on the PhagesDB (Protein) Server as well as the NCBI (Protein) Database.

Materials:

• Computer
• DNA Mastering Application
• NCBI Database (Protein)
• PhagesDB Database (Protein)
• Annotation Key
• GeneMark

Procedure:

• Opened DNA Mastering on the computer and opened Elesar file
• Auto-annotated and opened up frames view of the genes
• Opened up PhagesDB and NCBI Databases on separate tabs
• Copied the protein sequence from the genes (This case Gene 1) and inputted into the BLAST in both NCBI and PhagesDB
• Recorded the annotations needed (SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn)

Observations:

• This screenshot shows the annotations in the notes box for the gene. Not all annotations are filled in because time ran out

Conclusion/Next Steps:

• Learned more about DNA Mastering and the process needed to completely annotate and document the gene. With this new information, will be able to apply to the annotation of NapoleonB