Elsar BLAST Practice 1/28/19

Rationale: Learn basic annotation tools, and become familiar with BLAST NCBI database.

Procedure:  

• DNA Master was opened
• Elsar FASTA file opened and auto annotated
• The reading frames were examined and adjusted
• Genes 1 and 2 were selected to BLAST
• Results from BLAST were examined and used to start annotation
• Gene 14 of Elsar was chosen at random to BLAST-Start the gene in the notes
• the results of Gene 1 and 14 were posted on DNA Master

The procedure of the bLAST was used as a practice BLAST to familiarize with the NCBI BLAST results. These results allow one to compare the query DNA sequence to similar sequences of DNA, and potentially allows identification of conserved DNA in multiple genome.

Gene 14

SSC: CP: SCS: ST: BLAST-Start: hypothetical protein [Arthrobacter sp. ok362], gene 14, NCBI, Q :S, coverage, 5e-58 Gap: LO: RBS: F: SIF: SIF-Syn

Results: 

Conclusions: The video played in the lab helped a great deal on understanding the results of a BLAST gene. With the results of the BLAST, BLAST-Start was done on gene 14. The results from BLAST allow one to compare the query DNA sequence to similar sequences of DNA as one means of verifying one gene or multiple genes. This helps researchers understand the function of the gene and each protein.

Future plans: Learn the basics of annotation through the SEA: PHAGES website and from the lab TAs. BLAST all of the genes of Napoleon B and compare the results of the BLAST with different databases such as Genemark and the auto-annotation results.