1.28.19 BLAST Analysis

Rationale: The next step in learning how to accurately and scientifically annotate a genome was considered to be learning BLAST analysis techniques. Therefore, this laboratory session was dedicated to practicing BLAST analysis using the Elesar phage genome.

Procedure:

• Opened DNA Master
• Opened FASTA File Elesar
• Export –> Create Sequence from this entry only
• Genome –> Auto Annotate –> Annotate
• Copied Gene 5 protein sequence and inputted to NCBI BLAST database
• Analyzed Results

Results:

[Elesar, 5, NCBI, Query 1 to Subject 1, 99%, 0.0, terminase large subunit]

Conclusions: Since the query had a 99% coverage and an e value of 0.0, it was concluded that Gene 5 of Elesar was likely coding for the terminase large subunit. This is backed by BLAST evidence, as 97% of the amino acids were a direct match to the NCBI sequence and 98% of the amino acids could be considered positive.

Future Plans: In the next lab session, the full annotation process will be assembled and put together. At this point, it will be possible to practice annotations on Elesar, and finally later on NapoleonB.