Purpose and Rationale: Continue learning basics of phage annotation by comparing genes of Elesar to those found in BLAST results.

Procedure:

• Opened Phage Elesar in DNA Master, using saved dnam5 file
• Blasted Elesar Gene 1 in phagesdb.org and learned basics on comparing BLAST results to findings
• Found gene 1 to not have any matches in phagesdb.org

• BLASTed Gene 1 in the NCBI page, but still had no match
• Following instructions from the SEA PHAGE Annotation Key, added to the gene’s notes that there are no significant BLAST alignments.
• Continued reviewing the 15 Guidelines for Phage Annotation with the class and adding small notes in the same box as previous.                                                                                                    
• Worked to find the correct start to Gene 1 by comparing start codons, coding potential, and again BLASTing the potential new gene in phagesdb and NCBI. 
• Saved and closed the project.

Results: We have changed the location of the start of gene 1, and learned how to compare BLAST results to the query.

Future Plans: Continue annotating the genome of Elesar. Refine skills and learn how to use a computer.