Gene Annotation Intro and Template Setting

Date: 1-23-19

• Rational
  • The rational for this lab was to add a template to DNAMaster for autoannotation and understand the counting and significance of gaps and overlaps between gene sequences.
• Procedure
  1. DNA Master was opened.
  2. A template was added by going to File > Preferences > Local Settings > New Feature. ‘Insert template into notes during autoannotation’ was selected and a template code was pasted in the text box.
  3. The code “SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn” was added with the following meanings:
     • SSC: Start/Stop Coordinates
     • CP: Coding Potential
     • SCS: Start choice source
     • ST: Starterator
     • BLAST-Start: The best BLAST match for the gene
     • Gap: Gaps or overlaps
     • LO: Longest ORF, for genes with ~10 bp between start codon and upstream stop codon
     • RBS: Ribosome binding sites
     • F: Gene function
     • SIF-BLST,-HHPred,-Syn: Supporting information for the function
  4. Elesar was opened and autoannotated with this template saved.
• Results
  • The template appeared in the notes section of the autoannotated Elesar.
• Conclusion
  • Using the template allows for more accurate presentation of annotation information in the notes section.
• Future Plans
  • This template will be used for future annotations. As gene annotation experience is gained, it will be easier to recognize and analyze trends as well as correctly manually annotate gene sequences.