Rationale:

Learned how to evaluate reading frames, as well as how to calculate the gap or overlap between genes.

Materials:

• Laptop
• DNA Mastering Application
• Learning Manual Powerpoint

Procedure:

• Opened DNA Mastering on laptop
• Entered in updated template code from PowerPoint (In preferences tab)
• Auto-annotated Elesar and brought up the Open Reading Frames

Observations:

• The spreadsheet shows indicates the endpoints of the 5′ and 3′, which can be used to calculate the gap or overlap between genes

Conclusion/Next Steps:

• With this lab, learned more about DNA Mastering and how to operate the application. The next step(s) would be to auto-annotate Elesar