Purpose: The purpose of today’s lab was to learn more about auto annotations and reading frames.

Tools/Procedures:

Tools:

• DNA Master
• Elesar FASTA file

Procedures:

1. DNA master was opened
2. The template was updated and saved for future work. The new template is SSC: CP: SCS: ST: Blast-start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn:.
3. With the new preferences, Elesar was auto-annotated again.
4. Elesar’s ORFs were examined.

Results:

• Elesar was auto-annotated.
• ORFs were examined.

Conclusions:

Elesar was auto-annotated successfully. The process of learning how to use the DNA Master program was continued.

Future Work:

For future work, the next steps will be to learn how to manually annotate and begin analyzing the auto-annotation of Elesar. Also, the process of learning DNA Master will be continued.