Rationale: In order to aide in performing annotations this year, the software “DNA Master” was installed onto my laptop. The FASTA file of the phage Elesar was used to test whether the program was installed properly.

Tools:

1. WINE was used in order to help install the Windows version of the software on my mac, as it seemed to be more stable than the mac version.

2. PhagesDB was used to download the executable installer and the FASTA file for Elesar.

Procedure:
1. The executable installer was downloaded from the PhagesDB website as well as WINE.

2. WINE was installed and the executable was run through WINE to install DNA Master.

3. The FASTA file for Elesar was downloaded from PhagesDB and loaded into DNA Master.

4. An auto-annotation was run on the Elesar genome.

Results: The program successfully auto-annotated the genome of Elesar from the FASTA file provided.

Conclusion: DNA Master was successfully installed properly onto my laptop.

Future Work: Next I will practice annotation on the genome of Elesar using the help of the newly installed program. Once I have sufficiently practiced, I will start annotating the genome of NapoleonB.