16 January 2019 ✷ DNA Master

Rationale: DNA Master was downloaded onto personal computers in order to practice annotating DNA.

Procedure

• Elesar, a previously discovered phage within PhagesDB, had a recorded DNA sequence file that was downloaded and then annotated through DNA master.
• The preferences on DNA Master were updated to use different colors and base pairs that were to be included were updated; the program was also given permission to use the HHMI database.
• The FastA file for Elesar was opened and run through the program, producing the images seen below.

Observation, Results, Data

The genome of Elesar is larger than I had imagined, given the small size of a bacteriophage. The sheer length of its genetic code is impressive to see written out as it is in DNA master.

Interpretations, Conclusion, Next Steps

In the following lab periods, the genome of phages discovered within the 2018-19 cohort of Baylor Univerity’s SEAPHAGES group will be analyzed and annotated.