January 16th, 2019

Objectives: Install and understand basics of DNA Master annotation program.

Rationale: DNA Master is the program the lab is using for annotations, therefore I must have it and know how to use it.

Procedure:

• Downloaded DNA Master onto my personal computer
• Began configuring basic settings such as color and other preferences
• Enabled program to use TTG Codons as start codons, also in preferences
• Imported Phage Elesar from phagesDB online, as a fasta file
• Opened Elesar in DNA Master, and created a sequence from the Elesar entry only
• Selected Elesar on main page, and under the GEnome tab in the top portion of the screen, selected auto-annotate
• Hit error with Glimmer preferences, spent time to correct
• Annotated Elesar without BLAST-ing all genes.
• Completed lab time.

DNA Master after completing the steps detailed above

Future Plans: Continue following basic annotation instructions and learning the process of using DNA Master.