Rationale: To calculate the titer and determine whether or not a new plate has to be made to achieve a high titer. If not, flood each plate.

Procedure:

• The titer of each plate with phages present was calculated and determined if the titer was high enough to be considered with pure phage.
• The plate containing 1X concentration and 2X concentration were flooded with 8 mL of phage buffer than placed on the shaking incubator.

Results and Analysis:

Conclusion:

The titer of 1X concentration and 2X concentration were calculated and flooded.

Future Plans:

DNA Extraction