Rationale: To filter the flooded plates to obtain more lysate.  

Procedure: 

1. Created an aseptic zone by cleaning the table with Cidecon and 70% Ethanol, and using a ethanol burner.  
2. Added 5 mL of Phage Buffer into each of the plates, and set on the shaking device.  
3. The plates were shaken for an hour and five minutes. 
4. Using a syringe and filter, filtered the lysate from each of the plates into a 50 mL tube.  
5. The 50 mL tube was stored in the fridge.  

Observations/ Results: 

Control plate was not contaminated and plates were webbed.  

Obtained about 13 mL of lysate from the webbed plates.  

When picking up the plates from shaking device realized that the plates were wet and some of the liquid had possibly spilled, and therefore decreasing the amount of lysate that was obtained.  

Next Step/Conclusion: 

Next class we will make more webbed plates and try to create a higher titer plate of either 10^9 or 10^8.