Rationale:

A TEM grid will be prepared, so a TEM of Ferranti can be taken next time.

Procedure:

1. Placed a slip of parafilm paper inside a petri dish.
2. Pipetted 15 µL of the HTL, 20 µL of DI water twice, and 20 µL of uranyl acetate onto the parafilm.
3. Placed the copper mesh TEM disc in the HTL drop for 5 minutes, then in the DI water drop for 2.5 minutes, then in the other DI water drop for 2.5 minutes, then in the uranyl acetate drop for 1 minute and 15 seconds.
4. Blotted the copper mesh TEM disc with filter paper.
5. Stored the copper mesh TEM disc in A8 in the TEM grid.

Observations:

• The disc was placed shiny side down on all the drops.
• The lysate drop appeared to have a faint crystal goldish color. The DI water drops were clear. The uranyl acetate drop had a faint lime green tint. The picture below shows the drops.

• When placing the copper mesh TEM disc into the A8 spot, the disc crinkled up on itself.

Next Steps:

The TEM grid will be viewed under a TEM. If the TEM does not turn out well, another copper mesh TEM disc will be prepared to view the lysate. Also, the DNA extraction procedures will be completed.