Rationale:

To prep a TEM grid with my high titer lysate in order to image the phage it is composed of.

Procedures:

1. Transferred 15µL of NMN CEW Lysate 10^0 to a piece of parafilm in a plate.
2. Transferred 15µL of water onto the same piece of parafilm.
3. Repeated step two for an addtional dot of water.
4. Added one drop of Uranyl Acetate onto the same piece of parafilm.
5. Put a copper mesh TEM disc shiny side down onto the lysate drop for 5 minutes.
6. Transferred the disc to the first drop of water for 2.5 minutes.
7. Transferred the disc to the second drop of water for 2.5 minutes.
8. Transferred the disc to the drop of Uranyl Acetate for 1 minute.
9. Blotted the disc on filter paper and stored for later use with the microscope.

Observations:

There was some questioning regarding if we should have blotted the disc to remove the excess uranyl acetate.

Next Steps:

The next step will be to use the electron microscope to image the phage contained on the copper disc. Additionally, we will also begin the process of prepping the lysate for DNA extraction and analysis.