Rationale: The purpose of this lab is to create webbed plates to create more lysate at a higher titer.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit.
2. The titer from the spot titer test was determined to be 7.5 x 10^8.
3. Top agar was made for 5 plates. 10 ml LB and 112.5 ul of CaCl2 were added to a tube.
4. 10 ul of the 10^-4 dilution of lysate 1 was added to four tubes of 0.5 ml of arthrobacter and allowed to sit for 10 minutes.
5. 12.5 ml of 2x TA was added to the top agar solution and pipetted up and down to mix. 4.5 ml of the solution was added to each of the arthrobacter tubes and then poured directly onto plates labeled LIP 11-19-18 PA web. The rest of the solution was poured onto a plate labeled LIP 11-19-18 Control.
6. The plates were allowed to sit for 10 minutes and then inverted and stored in the incubator until the next lab.
7. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Bubbles were seen on the control plate

Interpretations/Next Steps:
The procedure was complete. The next step will be to flood the plates and collect the lysate.

Titer Test and Plates Made: