Results from Previous Experiment: Discovered our lysate to have a titer of 10^11.

Objectives: Complete TEM with the high titer lysate.

Procedure:

• Allocated Petri Dish and Parafilm
• Placed a strip of parafilm securely inside petri dish top
• Placed a drop of lysate, 2 separate drops of water, and a drop of Uranyl less on the parafilm
• Using precision tweezers, placed a copper grid, shiny side down, on the lysate for 5 minutes
• Moved copper grid to the first drop of water for 2.5 minutes
• Moved copper grid to the second drop of water for 2.5 minutes
• Placed copper grid on uranyl less (UL) for 1 minute, and quickly blotted an excess UL once remove
• Used tweezers to move copper grid into TEM Grid Holder

Future Plans: Complete TEM after thanksgiving break.