11/12/18

Rational:

To do a plaque assay for three dilutions in order to calculate the titer for lysate 7.

Procedure:

• Cleaned lab desk
• Put 90 ML of PB in to 3 microcentrifuge tubes
• Added 10 ML of the lysate to the first tube (10^-1)
• Added 10 ML of the 10^-1 lysate to the second (10^-2)
• Added 10 ML of the 10^-2 lysate to the second (10^-3)
• Added 10 ML of the lysate and the three dilutions to 4 tubes of .5 mL arthro and waited 10 min
• Put 10 mL of LB broth into a tube
• Added 112.5 ML CaCl2 and added 2 mL the TA mixture to each of the tubes of arthro
• Added 2.5 mL of TA to the dilutions and control
• Poured on plates and waited 15 min
• Put in incubator at 26 C at 3:40

Conclusion:

Next lab the titer of lysate 7 will be determined. If it is a high titer then TEM will be done with the lysate if it is not a new lysate will be prepared in order to get a high titer lysate.